EXPERIMENTAL RENAL GLOMERULOGRAPHY

¹ From the Surgical, Research, and Radiology Services, Veterans Administration Hospital, Oteen, North Carolina

An angiographic technique is described whereby the glomeruli of the canine kidney in the intact animal can be visualized consistently. The method involves the use of momentary renal circulatory arrest produced by inflating a balloon catheter in the abdominal aorta at the renal arterial orifice, with injection of radiopaque medium directly into the renal artery distal to the balloon through a second catheter. Fine grain film, ultra-detail intensifying screens, and direct roentgenographic magnification to 3 times actual size are also employed. Confirmation of the nature of the tiny round discrete shadows distributed radially and preferentially in the cortex was obtained by comparing the angiograms with those obtained using ethiodol as the contrast medium. The distribution of the ethiodol both by microradiography and histology using fat stains was almost entirely in the glomeruli. The toxicity of the method was cursorily assessed by blood urea nitrogen determinations, I¹³¹ renograms and histology, and was found to be low.

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