AJR InPractice
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Abstract Freely available
Right arrow Figures Only
Right arrow Full Text (PDF)
Right arrow Clips
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yam, C.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yam, C.-S.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
DOI:10.2214/AJR.05.1796
AJR 2006; 187:W93-W101
© American Roentgen Ray Society


Computers in Radiology

Preparation of Digital Movie Clips for Online Journal Publication

Chun-Shan Yam1

1 Department of Radiology, Beth Israel Deaconess Medical Center, 330 Brookline Ave., Boston, MA 02215.

Received October 14, 2005; accepted after revision January 23, 2006.

 
Address correspondence to C.-S. Yam (csyam{at}caregroup.harvard.edu).

WEB

This is a Web exclusive article.


Abstract
Top
Abstract
Introduction
Definitions
Discussion
References
 
OBJECTIVE. This article presents general guidelines for preparing movie clips for online journal publication.

CONCLUSION. As more and more radiology journals establish an online presence, radiologists wishing to submit journal articles with movie clips need to understand the electronic submission process. Viewing a movie clip via an online journal is different from viewing one with PowerPoint using a local desktop computer because the movie file must first be downloaded onto the client computer before it can be displayed. Users thus should be cautious in selecting movie format and compression when creating movie clips for online journals. This article provides step-by-step demonstrations and general guidelines for movie format and compression selections.

Keywords: computer movie clips • research


Introduction
Top
Abstract
Introduction
Definitions
Discussion
References
 
Most radiology journals today are available online in addition to their paper versions. For many academic radiologists, searching journal databases, reading current articles, browsing upcoming issues, and submitting manuscripts electronically are already a part of their daily work flow. A major advantage of radiology online journals (e-journals) is the ability to present 3D/4D data sets using movie clips. With today's widely available MDCTs and fast workstations, many functional studies are now performed routinely, and, therefore, more movie clips are being used in radiology presentations. For many academic radiologists, however, preparing movie clips is a relatively new and potentially intimidating procedure. In this article, I provide general guidelines for preparing and submitting movie clips to a radiology journal such as the American Journal of Roentgenology (AJR).

The information here is aimed specifically at creating Internet-compatible movie clips for online journal publication, not PowerPoint presentations. Using movie clips in PowerPoint requires extra considerations regarding movie format and compression algorithm selections. I offered detailed guidelines for preparing movie clips for PowerPoint presentations in my previous article [1].


Definitions
Top
Abstract
Introduction
Definitions
Discussion
References
 
Movie Clip
Although "movie clip" is defined in many different ways in today's multimedia and animation industry, only one straightforward meaning is relevant for radiology presentations. A movie clip is a short sequence of images used to show the underlying functional and anatomic characteristics of a clinical or research study. Depending on applications, a movie clip can be as simple as a stack of 2D axial images or as complicated as a cine loop of 3D fly-through virtual colonoscopy images. Movie clips are often created from common image files such as PNG, BMP, JPEG, and TIFF. The two most important parameters for creating movie clips are the playback speed and the movie compression.

Playback Speed
The playback speed of a movie clip is measured in frames per second (fps). If a movie clip contains 50 images with a playback speed of 10 fps, the total playing time for the entire movie is 5 seconds. Depending on applications, frame rates between 10 and 30 fps should be optimal for most Internet-based applications, including e-journals.

Movie Compression: Codec
A codec is a software algorithm used to compress and decompress digital videotape or audio files. Using a codec in movie clips reduces file size. This capability is especially important for today's multimedia applications over the Internet where transmission of huge data files is still the most common bottleneck issue. Currently, many of the movie clips are suitable for online journal publications (e.g., MS AVI Videotape, MPEG Movie, and QuickTime Videotape); however, some require specific movie viewers such as Windows Media Player (Microsoft) and QuickTime Player (Apple). For instance, most of the QuickTime movies (*.mov) are not supported by Windows Media Player, and some AVI movies (*.avi) cannot be viewed by both Macintosh and PC without installing additional third-party codec(s) [1]. Be careful not to choose uncommon settings for your movie clips. Similarly, MPEG (MPEG-1), a traditional movie format developed by Motion Picture Experts Group, was once a common format used by many media applications but is now a mystery to many people because of the wide range of its derivatives (i.e., too many different kinds of MPEG). For this reason, I conducted a compatibility study among file formats, videotape codec, movie viewers, and computer platforms. Tables 1, 2, 3 present the results of this study. You can use these tables as general guidelines for selecting movie format and codec for your movie clips.


View this table:
[in this window]
[in a new window]

 
TABLE 1: QuickTime Format (*.mov) Results of Web-Viewing Compatibility Study

 

View this table:
[in this window]
[in a new window]

 
TABLE 2: AVI Format (*.avi) Results of Web-Viewing Compatibility Study

 

View this table:
[in this window]
[in a new window]

 
TABLE 3: MPEG Format Results of Web-Viewing Compatibility Study

 

Depending on journals and publishers, movie format and codec requirements for movie clip submissions may vary. As a good practice, always refer to the submission guidelines provided by the journal. For an example, Appendix 1 provides the compatible movie file formats and other types of supplemental data for online publications in AJR.


View this table:
[in this window]
[in a new window]

 
APPENDIX I: Possible File Formats Used as Supplemental Data for AJR Online Submission

 

Movie-Editing Software
Many desktop applications can be used for creating movies clips: Platypus Animator (Snapfiles), Photo Slide Show (DigitByte Studio), and QuickTime Pro (Apple) [2-4]. For demonstration, I describe the essential steps for creating and compressing movie clips from common image files using QuickTime Pro, which is a cross-platform program compatible for both PC and Macintosh systems (currently retails for approximately $29.99). I discussed detailed information for installing and using QuickTime Pro in a previous article [1]. Although the process steps described here are based on this particular application, the basic approach to creating movie clips can be applied to other editing software.

Note that the editing software described is only required for creating the movie clips, not for viewing them. Online readers do not need to purchase any editing software to view movie clips. Common movie viewers such as Windows Media Player and QuickTime Player are adequate for viewing movie clips. Selection of movie viewers is discussed later.

Demonstrations
For demonstration, I describe common sources for movie clips, the basic technique for optimizing movie format and file size, and the essential process for uploading movie files, in this case, to the AJR Rapid Review Web site. Three types of movie clips—QuickTime Videotape (.mov), MPEG Movie (.mpg), and MS AVI Videotape (.avi)—are used for this demonstration. All these movie formats are Internet compatible and acceptable for online publication in AJR (Appendix 1). Note that although I use CinePak as codec for the first two sample movies, other codec(s) can also be selected (Tables 1, 2, 3). In my previous article, I explained that most of the common codec(s) produce similar compression ratios [1].

Sample Movie 1
This first example shows the essential steps for creating a movie clip in QuickTime Videotape format from a series of 2D images. This approach is most appropriate for academic radiologists who need to create movie clips without special equipment such as an image-processing workstation. The movie clip used in this example is a simple cine loop of 92 axial images obtained from a contrast-enhanced CT angiography study using a 64-MDCT scanner (Aquillion, Toshiba). In this example, the study images were stored on a patient CD directly from the scanner.


Figure 1
View larger version (71K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 1 Screen captures of QuickTime Pro showing process for loading sequence of PNG images from folder.

 


Figure 2
View larger version (62K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 2 Screen captures of QuickTime Pro showing process for exporting movie clip in QuickTime Movie format: sample movie clip 1: axial.mov available online at www.ajronline.org.

 
Although the patient CD contains both DI-COM (Digital Imaging and Communications in Medicine) and PNG images, only the PNG images are used. However, if either the storage media or the image format is not compatible with the desktop computer (e.g., DICOM images in magnetooptic disks), a format conversion is needed. Detailed instructions for conversion are available in my previous article [5]. The source images must be converted into a common picture format (e.g., PNG, BMP, JPEG, TIFF, etc). While preparing the images, be careful not to include patient information on the images. Similarly, adjust the brightness and contrast of the images, if necessary, during this step. Based on my experience, any kind of modifications after the movie is created will be cumbersome. For this example, I simply turned off the image annotations before exporting the images onto the patient CD. However, if patient information is embedded, such as in sonographic images, you must remove it before creating the movie clip [6].

In QuickTime Pro, creating a movie clip from a sequence of images is straightforward. Select the Open Image Sequence function from the File menu (Fig. 1A). At the Open File window, navigate to the patient CD and select one of the PNG images, and then select the OK button (Fig. 1B). At the next screen, select 15 fps as the frame rate and click the OK button to load the images (Fig. 1C). By default, QuickTime Pro loads all images with equal dimensions within the same folder. After loading all 92 images into a new movie window (Fig. 1D), use the control buttons at the bottom to examine the movie content. To save the movie, select the Export function from the File menu, and then select Movie to QuickTime Movie at the Export drop-down menu (Fig. 2A). Next, click on the Options button to open the Movie Settings window (Fig. 2B), and then click on the Settings button to open the Compression Settings window (Fig. 2C). In this example, I used CinePak as the compression codec, Millions of Color as the color profile, and Best for the videotape quality. Click the OK button to start the movie creation process. Depending on your operating system and hardware configurations, the processing time may range from seconds to minutes. Using my desktop PC (Dell Precision Workstation, 1 GB RAM, 200 GB hard drive) running Windows XP Professional (Microsoft), the movie clip was created in approximately 10 seconds. After the movie was created, I opened the movie clip with QuickTime Pro to spot-check the content (Supplemental Data 1, "axial.mov"). Select the Show Movie Info function from the Window menu to view the detailed movie parameters (Fig. 2D). The file size of this movie clip is 5.9 Mb.


Figure 3
View larger version (49K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 3 Screen captures of QuickTime Pro showing detailed information for sample movie clip 2: volume.avi available online at www.ajronline.org.

 


Figure 4
View larger version (62K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 4 Screen captures of QuickTime Pro showing detailed information for sample movie clip 3: cathlab.mp4 available online at www.ajronline.org.

 
Sample Movie 2
Instead of creating movie clips from individual images as described in the first example, movie clips can also be generated directly from image processing workstations, such as Advantage Window (GE Healthcare), Vitrea (Vital Images), Virtuoso (Siemens Medical Solutions), and Insight (Neo Imagery Technologies). Many of the current workstations are fast and fully equipped with sophisticated image processing functions such as volume rendering. Based on my experience, movie clips generated from these workstations are already in common desktop formats. Also, with the new operating systems (Windows 2000 and XP, Microsoft), workstations allow movie clips to be exported directly to portable media such as a USB storage drive. However, depending on applications and vendors, file format and compression of the exported movie clips may vary [1]. Often, conversion and optimization of the movie clips are required to meet the publisher's requirements. In this example, I show the use of simple QuickTime Pro functions for verifying and optimizing a sample movie clip obtained from a workstation. This sample movie contains 3D volume-rendered images created from the same CT data set as in the previous example.

The axial images were sent from the scanner to a nearby workstation using the DICOM transfer protocol for generating a 3D model. A 360° snapshot movie of the model was then exported as a movie clip onto a portable USB drive. Depending on applications and/or user preferences, creating 3D images from a workstation may be more complex and labor intensive than this example.

After transferring the movie clip from the workstation onto my PC, I checked the movie format and file size using my editing software with the following steps. Select Open Movie in New Window function from the File menu to load the movie clip. Select the Show Movie Info function at the Window menu to display the movie parameters: This movie clip was created in noncompressed AVI format. Without compression, the file size of this movie clip is nearly 96 Mb. Although this large file is still acceptable for PowerPoint presentation using a well-equipped laptop computer, it is definitely too large for online journal publications. The easiest solution for reducing file size is to apply a compression. Select the Export function from the File menu, and then select the Movie to AVI for the Export option. Click on the Options button to open the AVI Settings window, and then click on the Settings button to open the Compression Settings window. Enter the same parameters as in the previous example (i.e., CinePak, Millions of Colors, and Best). Click the OK button to start the conversion process. The file size of the converted movie clip is reduced to 4.5 Mb (Fig. 3, Supplemental Data 2, volume.avi).

Similar to the previous example, be careful not to display any patient information in the movie clip. Based on my experience, disabling on-screen annotations at the workstation while preparing the movie clip is the most effective and easiest way to prevent this issue.


Figure 5
View larger version (69K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 5 —A, Screen capture of online AJR search page showing results of article search using keyway avi. In this search result, two articles contain movie clips [7, 8]. Web links for movie clips are located below PDF link. B, Screen capture of full-text Web page showing same Web links.

 
Sample Movie 3
Another common source of movie clips is from outside radiology (e.g., the cardiac catheterization laboratory, operating room, and research laboratory). The archiving media and formats of these movie clips may vary; however, in most of today's clinical settings, movie clips are usually created in common movie formats. In this example, I use a movie clip of a prestent placement obtained from the catheterization laboratory for the same patient as in the previous examples. The movie clip was created as a MPEG Movie (MPEG-4), a format compatible with AJR. Because the file size of this movie clip is already small (0.5 Mb), conversion was not necessary (Fig. 4, Supplemental Data 3, cathlab.mp4).

Uploading Movie Files
Uploading movie files to the online AJR Rapid Review Web site is similar to uploading TIFF images. However, movie files must be uploaded on the first page as supplemental data on the submission form. Currently, up to three supplemental data files can be submitted. In my case, I uploaded the three sample movie clips (i.e., axial.mov, volume.avi, and cathlab.mp4) as supplemental data files. Similar to figure legends, I provided a separate index page in the manuscript for descriptions of each of the supplemental files. This index page is usually searchable and makes the supplemental data accessible without having to go through the actual article (Trafton D, personal communication).

Viewing Movie Clips
Movie clips are available for viewing only on the online AJR journal Web page, not the PDF version. The Web links for movie clips are provided in two different places. At the Search Result page, the Web link appears on the right of the Search Result column with descriptions of the data file (e.g., "avi file for Fig. n") (Fig. 5A) [7, 8]. The same Web link will also appear at the top right of the full article Web page (Fig. 5B). For this particular article [7], three AVI movies using "MS Videotape 1" codec are provided—1b.avi (4.6 Mb), 1d.avi (4.2 Mb), and 1e.avi (4.4 Mb).

Movie clips are viewed in the same way as viewing the PDF article by clicking on the PDF Web link. However, unlike PDF files that must be opened in Acrobat Reader (Adobe), movie clips are displayed in a movie viewer. Depending on your computer platform, operating system, Web browser setting, and the movie clip format, different viewers may be launched (see Tables 1, 2, 3).

If the movie viewer is not present but required for a particular movie clip, installation of the viewer is necessary. For instance, if a movie clip was created in QuickTime (CinePak codec) and your PC (Windows XP) does not have the QuickTime Player installed, the Web browser will prompt you to install the QuickTime Player (Fig. 6A). For this particular case, click on the Install button and then follow the screen instructions to complete the installation. This may also happen to Windows Media Player, especially when a newer version is required. Based on my experience, installing this type of media application does not generally harm the computer. However, some companion applications such as iTune for QuickTime Player and MSN Messenger for Windows Media Player may be installed automatically. If the companion application is not useful, remove it. Go to Start, then Settings, Control Panel, and Add and Remove Programs. Select the item, and then click the Remove button to remove the application. Also, depending on your Web browser settings, some movie viewers (such as Windows Media Player) can be embedded within the browser window as a Media Bar (e.g., Internet Explorer SP1). To restore the viewer back to a standalone application, the browser settings must be changed. In Internet Explorer, click the Media icon on the toolbar to open the Media Bar. At the bottom of the Media Bar pane, select Media Options, point to Settings, and then click Play web media in the Bar to clear the check mark (Fig. 6B).


Figure 6
View larger version (68K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 6 —A, Screen capture of Internet Explorer 6.0 SP2 (Windows XP Professional) showing message box prompting for installation of QuickTime Player 6.0. B, Screen capture of Internet Explorer 6.0 SP1 (Windows 2000 Professional) showing Internet Options window for restoring Windows Media Player 9.0 as standalone application.

 

Choosing Movie Viewers
Windows Media Player currently is the default movie viewer for PC, and QuickTime Player for Macintosh. These two applications are similar in terms of viewing movie clips (i.e., they both provide VCR buttons at the bottom for controlling movie playback). However, some noticeable differences are apparent in the user interface. First, Windows Media Player allows a full-screen view; QuickTime Player does not (Fig. 7A). Second, QuickTime allows real-time scrolling of the movie frames; Windows Media Player does not (Fig. 7B). In other words, when the timeline button is dragged, QuickTime provides instantaneously update of the movie similar to scrolling through a stack of CT images in PACS. In addition to these two viewers, many other applications (shareware or freeware) can also be used for viewing movie clips. Real Player (Real Networks) is one example. This application is similar to the other two but with a different user interface. You can download the free versions of each of these three applications to evaluate them [9-11]. Note: Installing Windows Media, QuickTime, and Real Players will not harm the computer but only guarantees that more movie formats can be viewed (Tables 1, 2, 3).


Figure 7
View larger version (90K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 7A Screen capture of Windows Media Player 9.0 (Windows XP Professional) showing movie clip downloaded from AJR Web site [7].

 

Figure 8
View larger version (98K):
[in this window]
[in a new window]
[as a PowerPoint slide]
 
Fig. 7B Screen capture of QuickTime Player 6.0 (Windows XP Professional) showing movie clip downloaded from AJR Web site [7].

 
File Size Reduction
Viewing a movie clip via an online journal is different from viewing a movie clip from PowerPoint using a local desktop computer because the movie file must be downloaded first onto the client computer before it can be displayed. Although downloading large data files is becoming less of an issue at many workplaces where high speed Internet is generally available, it is still a problem for some locations where slow connection speed (e.g., a dial-up modem) is the only option. For instance, at a workplace with a T1 connection (1.5 Mbps or 0.2 Kbps [megabits or kilobits per second]), it will take approximately 30 seconds to download a 5.9 Mb file (e.g., axial.mov, sample movie 1), and 3 sec for a 0.5 Mb file (e.g., cathlab.mp4, sample movie 3). However, for dial-up connections (56 Kbps), the downloading time will be 27 times longer (i.e., 1,500 Kbps/56 Kbps = 27). Although high-speed Internet is commonplace in many locations (e.g., my home connection speed is 7 Mbps [download], Comcast cable modem), keeping the movie file size down is always a good practice. For instance, the original movie file size discussed in Sample 2 was 96 Mb, which is not acceptable. Here are several options for reducing file size:

Based on my experience, the first four options are most appropriate for reducing file size without sacrificing the output resolution. Although currently, no limitations on file size for movie clip submission to AJR are cited, I believe a file size of 5-6 Mb should be appropriate.


Discussion
Top
Abstract
Introduction
Definitions
Discussion
References
 
As more and more radiology journals establish an online presence, radiologists wishing to submit journal articles with movie clips must understand the electronic submission process in terms of compatible file formats and size restrictions. In this article, common sources for obtaining movie clips from typical radiology settings are discussed as well as techniques for optimizing movie format and file size. The rule of thumb in preparing movie clips is to use a common format and keep the file a reasonable size. General guidelines for selecting movie format and compression codec are provided (Tables 1, 2, 3).

Similar to the increasing trends of online journal submissions, many radiologic societies such as the American Roentgen Ray Society (ARRS), European Congress of Radiology (ECR), and Radiological Society of North America (RSNA) are now steadily converting scientific exhibits and posters into an electronic presentation format at their annual meetings. Although this format is similar to the traditional desktop PowerPoint projection, the presentation mechanism is significantly different. Presenters should be cautious when using movie clips in their presentations during this format shift. Using incompatible movie clips or having broken links to the movie files may cause unexpected disasters in their presentations. For this particular reason, I will discuss the use of animated GIF files and Macromedia Shockwave Flash in my next article.

Although animated GIF and Macromedia Shockwave Flash are not technically categorized as movie clips, they both have the capabilities for displaying dynamic data sets (multiple images). Unlike traditional movie clips (i.e., AVI, QuickTime, and MPEG), these two special types of media files are embedded within the PowerPoint presentation file, not "linked." With this advantage, dynamic data sets can be securely displayed in PowerPoint without the common issue of missing file links for movie clips. These two types of files are most appropriate for e-presentation and desktop projection.


References
Top
Abstract
Introduction
Definitions
Discussion
References
 

  1. Yam C-S, Kruskal J, Larson M. Using movie clips in PowerPoint presentations. Part 1, compatibility issues. AJR2005; 185:1074 -1078[Abstract/Free Full Text]
  2. Snapfiles home page: www.snapfiles.com/get/platypusani.html
  3. DigitByte Studio home page: www.321slideshow.com/contact.htm
  4. Apple Computer home page: www.apple.com/
  5. Yam C-S, Sitek A, Raptopoulos V, Larson M. A simple method for extracting DICOM images from a magnetooptic disk. AJR2004; 183:529 -533[Abstract/Free Full Text]
  6. Yam C-S, Kruskal J, Larson M. Using movie clips in PowerPoint presentations. Part 2, movie editing, AJR2005; 185:1371 -1376[Abstract/Free Full Text]
  7. Lawler LP, Ney D, Pannu H, Fishman EK. Four-dimensional imaging of the heart based on near-isotropic MDCT data sets. AJR2005; 184:774 -776[Free Full Text]
  8. Lee T, Tsai I-C, Tsai W-L, Jan Y-J, Lee C-H. Bronchogenic cyst in the left atrium combined with persistent left superior vena cava: the first case in the literature. AJR 2005;185 : 116-119[Free Full Text]
  9. QuickTime Player download page: www.apple.com/quicktime/
  10. Media Player download page: www.microsoft.com/downloads/search.aspx?displaylang=en
  11. Real Player download page: www.real.com/

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Am. J. Roentgenol.Home page
C.-S. Yam
A solution for using dynamic data sets in electronic presentations.
Am. J. Roentgenol., August 1, 2006; 187(2): W218 - W226.
[Abstract] [Full Text] [PDF]


This Article
Right arrow Abstract Freely available
Right arrow Figures Only
Right arrow Full Text (PDF)
Right arrow Clips
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yam, C.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yam, C.-S.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS